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Single-cell suspension is required for flow cytometry assays. Thus the adherent cell lines and tissue samples require processing into single-cell suspension before flow cytometry analyzed. A number of protocols are available and involved in mechanical dissociation or enzymatic digestion of the sample. Note: When an enzymatic digestion is carried out, incubation has to be carefully monitored. https://www.creative-diagnostics.com/flow-cytometry-protocol-sample-preparation.htm
About FC Protocol - Sample Preparation:
Single-cell suspension is required for flow cytometry assays. Thus the adherent cell lines and tissue samples require processing into single-cell suspension before flow cytometry analyzed. A number of protocols are available and involved in mechanical dissociation or enzymatic digestion of the sample. Note: When an enzymatic digestion is carried out, incubation has to be carefully monitored. https://www.creative-diagnostics.com/flow-cytometry-protocol-sample-preparation.htm
Category: Biotechnology
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